Location: Home >
Products >
SF-2004
SF-2004 Series of Stopped-Flow Instruments
| Contents |
|
|
 |
| Computer-Aided Design |
The KinTek Stopped-Flow has been computer designed, starting at the very
heart of the instrument - the drive system and the observation cell to provide
an integrates system that performs flawlessly. Easy to use for the
novice, and yet powerful for the most experienced professional.
The KinTek SF-2004 features unprecedented precision, reliability, and ease of use.
|
| Your
choice! Servo Motor or Pneumatic Cylinder Drive System |
 |
You can choose to have a SF-2004 Stopped-Flow with a powerful, computer-controlled servo
motor drive which provides precise and reproducible mixing of 2 or 3 solutions.
The motor is digitally controlled with a high torque, low inertia, brushless motor
and high performance, digital power supply to provide essentially instantaneous
starts and stops of the drive syringes. A major advance over older
stepper motor technology, the servo motor affords repeated or sequential mixing
to be performed with precision and ease, completely under software control. The
KinTek motor drive system provides the utmost in performance and reliability.
Alternatively, the researcher can now choose the SF-2004-AF Pneumatic Stopped-Flow. This instrument utilizes a computer-controlled
pneumatic drive cylinder. The pneumatic drive cylinder forces the mixing
of reactants against a stopping syringe. A set of solenoid valves and
a small drive cylinder function together to automatically empty the stop
syringe after each shot so that repeated or sequential mixing can be performed
with ease, completely under software control. All other aspects of the SF-2004 Pneumatic Stopped-Flow offer the same high quality
components as our SF-2004.
For ease of use and to help in the expulsion of air bubbles, the SF-2004
series of stopped-flows use a solid vertical drive mount. This arrangement
places the sample syringes in a position where they are easy to load and
to observe through the transparent water jacket during the course of an
experiment. Various sample chambers can be easily interchanged or removed
for cleaning
|
| Fluorescence - Absorbance Cells |
| The SF-2004 series of Stopped-Flow instruments uses interchangeable
observation cells. The user can select from a variety of cells to optimize
data collection to meet special needs. Each cell comes complete with syringe
assembly and integral sample loading valve, so changing the observation
cell is easy.
A choice of four observation cells is available and each provides not
only optimal fluorescence measurements but also unique features, which
can greatly improve data, collection under different conditions. All four
cells can record either absorbance or fluorescence or both simultaneously. An optional CCD array
detector allows spectra to be recorded every msec for truly outstanding performance.
|
Observation Cell
|
| Dead Time for Mixing |
|
The SF-2004 series of KinTek stopped-flows have a guaranteed dead time
of less than 1 msec. View the data!
With its precision, high torque servo
motor drive, the performance of the SF-2004 is repeatable and reliable
year after year. The SF-2004 also has excellent performance reliability
due to the precision manufacturing of all parts
and with proper care will give many years of service.
Some of our observation cells use Berger Ball mixers to provide efficient
mixing with minimal back-pressures. In others, we use our proprietary
microjet mixers. In both cases, test reactions have proven that mixing
is completed in the KinTek stopped-flow before the sample enters the cell.
|
|
Sample Loading Valve |
| A unique two-position valve is used to allow easy and efficient sample
loading. In one position, the three syringes are connected to their individual
sample load ports. In the other position, the three syringes can be driven
to force the mixing of reactants into the observation cell. Optical sensors
read the position of the sample load valve and tell the computer whether
the valve is in the proper position for loading or firing. The computer
will not fire a shot if the valve is in the wrong position. This safety
feature further conserves precious samples. |
|
| Sample Volume |
|
Designed to conserve precious samples, the KinTek SF-2004 requires only
20 microliters of each reactant per shot. Moreover, by minimizing the
volume of the sample lines connecting the loading ports to the sample
syringes, the instrument requires a small load volume. Typically, only
50 microliters of reactant is expended in loading each syringe.
|
| Three Syringe - Double Mixing Experiments |
 |
The KinTek stopped-flow comes with three drive syringes. In the normal
mode of operation, only two syringes are connected and used to drive the
mixing of two reactants. Alternately, the third syringe can be connected
to perform a double mixing experiment. Two reactants are mixed and then
flow into a delay line. The motor then stops for the desired reaction
time and then on the next push, the solution is mixed with the third reactant
and forced into the observation cell. The reaction time is under precise
computer control, allowing reactant times as short as 5 msec and up to many minutes.
Double mixing experiments allow new experiments to explore reaction mechanisms
that cannot be accomplished with other commercially available instruments.
|
|
T-Format Fluorescence Anisotropy! |
|
The SF-2004 can be easily and economically equipped to perform fluorescence
anisotropy measurements. A second optional PMT is added to the instrument
along with an easy to use filter set and the instrument becomes an optimal
anisotropy instrument. Check out the data!
|
|
Software |
| KinTek SF-2004 is noted for the ease of use and reliability of its software.
Running under Windows XP, the SF-2004 operating system utilizes pull down
menus to access all of the computer-controlled instrument setup, data collection,
analysis and plotting. Data and computer-generated fits are displayed on
the video terminal and can be printed on any printer supported by Microsoft
Windows. Because the system uses Windows XP, data is easily transported
to other analysis and graphics packages.
Don't buy a stopped-flow using an outdated computer system.
With the KinTek SF-2004 you get the world-wide standard DELL PC and have access
to the wealth of computer programs for data analysis and presentation.
The SF-2004 system software allows data to be easily exported to other
programs in ASCII spreadsheet format - at the click of a mouse!
|
| Computer Control |
|
The KinTek SF-2004 is entirely under computer control, making the operation
of the instrument easy and convenient to use. The computer controls the
instrument drives system, the detector gain and checks to see that the
sample load valve is in the right position. It is also programmed to assist
in a simple calibration of the instrument. The instrument and software
have been integrated into a system that is easy and convenient to use,
leaving the operator free to concentrate on the data collection and analysis.
|
| Data Collection and Analysis |
| A 3 GHz Pentium 4 and 16-bit 413kHz A/D converter afford rapid data collection
and analysis. Data fitting is accomplished by nonlinear regression. Data
can be fit to one, two or three exponentials or to a burst equation.
Data can also be fit by computer simulation directly to the reaction
mechanism by global fitting methods using KinTekSim.
Data can also be easily exported for import into the data fitting and graphics presentation program
of your your choice.
|
 |
| Light Source for Optimal Signal |
|
Optimal signal-to- noise is provided through several unique advances.
A high intensity, stable arc lamp source illuminates the sample using
a parabolic mirror and lens-free optics to provide optimal throughput
to a monochromator. The optical path is free of lenses, which would limit
light throughput. The electronic circuits have been designed to provide
the most noise-free amplification possible.
|
 |
| Specifications |
| Wavelength range: |
220 - 800 nm.
|
| Wavelength accuracy: |
1 nm.
|
| Stray light: |
less than 0.02%
|
| Spectral bandwidth: |
Continuously variable slit width providing a bandwidth of
4 nm/mm of slit width.
|
| Temperature range: |
0 - 70° C range of temperature is maintained by circulating
water bath (not provided).
|
| Dead time |
Less than 1 msec.
|
| Syringe volume & range: |
5 ml standard, 0.25, 0.5, 1.0, 2.0 also available.
|
| Computer operating system: |
Windows XP
|
| A/D Converter: |
416 kHz, 16-bit, 8 channels |
| Physical Dimensions |
| Component |
Width x Depth x Height |
Weight |
| Stop-flow stand: |
36 x 26 x 68 cm. |
17.5 kg. |
| Motor controller |
43 x 35 x 22 cm. |
11.2 kg. |
| Light Source: |
80 x 26 x 13 cm. |
10.5 kg. |
| Lamp power supply: |
28 x 30 x 11 cm. |
7.8 kg. |
|