SF-2004 Series of Stopped-Flow Instruments
- Choose either a Servo Motor Drive or Pneumatic Cylinder Drive System.
- Fluorescence - Absorbance Cells
- Sample Loading
- Double Mixing Experiments
- Fluorescence Anisotropy!
- Software and Computer Control
- Data Analysis
- Specifications
- Request a Quote
Computer-Aided Design
The KinTek SF-2004 Stopped-Flow has been computer designed starting at the very heart of the instrument, the drive system and the observation cell, to provide an integrated system that performs flawlessly. It is easy to use for the novice, and yet powerful for the most experienced professional.
The KinTek SF-2004 features unprecedented precision, reliability, and ease of use.
Servo Motor Drive System
You can choose to have a SF-2004 Stopped-Flow with a powerful, computer-controlled servo motor drive that provides precise and reproducible mixing of 2 or 3 solutions. The motor is digitally controlled with a high torque, low inertia, brushless motor and high performance, digital power supply to provide essentially instantaneous starts and stops of the drive syringes. A major advance over older stepper motor technology, the servo motor affords repeated or sequential mixing to be performed with precision and ease, completely under software control. The KinTek motor drive system provides the utmost in performance and reliability.
For ease of use and to help in the expulsion of air bubbles, the SF-2004 series of stopped-flows use a solid vertical drive mount. This arrangement places the sample syringes in a position where they are easy to load and to observe through the transparent water jacket during the course of an experiment. Various sample chambers can be easily interchanged or removed for cleaning.
Fluorescence - Absorbance Cells
The SF-2004 series of stopped-flow instruments uses interchangeable observation cells. The user can select from a variety of cells to optimize data collection to meet special needs. Each cell comes complete with syringe assembly and integral sample loading valve, so changing the observation cell is easy.
A choice of four observation cells is available and each provides not only optimal fluorescence measurements but also unique features, which can greatly improve data collection under different conditions. All four cells can record either absorbance or fluorescence or both simultaneously. An optional CCD array detector allows spectra to be recorded every millisecond for truly outstanding performance.
Dead Time for Mixing
The SF-2004 series of KinTek stopped-flows have a guaranteed dead time of less than 1 millisecond. View the data! With its precision, high torque servo motor drive, the performance of the SF-2004 is repeatable and reliable year after year. The SF-2004 also has excellent performance reliability due to the precision manufacturing of all parts and with proper care will give many years of service. Some of our observation cells use Berger Ball mixers to provide efficient mixing with minimal back-pressures. In others, we use our proprietary microjet mixers. In both cases, test reactions have proven that mixing is completed in the KinTek SF-2004 Stopped-Flow before the sample enters the cell.
Sample Loading Valve
A unique two-position valve is used to allow easy and efficient sample loading. In one position, the three syringes are connected to their individual sample load ports. In the other position, the three syringes can be driven to force the mixing of reactants into the observation cell. Optical sensors read the position of the sample load valve and tell the computer whether the valve is in the proper position for loading or firing. The computer will not fire a shot if the valve is in the wrong position. This safety feature further conserves precious samples.
Sample Volume
Designed to conserve precious samples, the KinTek SF-2004 requires only 20 microliters of each reactant per shot. Moreover, by minimizing the volume of the sample lines connecting the loading ports to the sample syringes, the instrument requires a small load volume. Typically, only 50 microliters of reactant is expended in loading each syringe.
Three Syringe - Double Mixing Experiments
The KinTek SF-2004 Stopped-Flow comes with three drive syringes. In the normal mode of operation, only two syringes are connected and used to drive the mixing of two reactants. Alternately, the third syringe can be connected to perform a double mixing experiment. Two reactants are mixed and then flow into a delay line. The motor then stops for the desired reaction time and then on the next push, the solution is mixed with the third reactant and forced into the observation cell. The reaction time is under precise computer control, allowing reactant times as short as 5 millisecond and up to many minutes. Double mixing experiments allow new experiments to explore reaction mechanisms that cannot be accomplished with other commercially available instruments.
T-Format Fluorescence Anisotropy!
The SF-2004 can be easily and economically equipped to perform fluorescence anisotropy measurements. A second optional PMT is added to the instrument along with an easy to use filter set and the instrument becomes an optimal anisotropy instrument. View the data!
Software
KinTek SF-2004 is noted for the ease of use and reliability of its software. Running under Windows XP, the SF-2004 operating system utilizes pull-down menus to access all of the computer-controlled instrument setup, data collection, analysis and plotting. Data and computer-generated fits are displayed on the video terminal and can be printed on any printer supported by Microsoft Windows. Because the system uses Windows XP, data is easily transported to other analysis and graphics packages.
Do not buy a stopped-flow using an outdated computer system. With the KinTek SF-2004 you get the world-wide standard DELL PC and have access to the wealth of computer programs for data analysis and presentation available. The SF-2004 system software allows data to be easily exported to other programs in ASCII spreadsheet format — at the click of a mouse!
Computer Control
The KinTek SF-2004 is entirely under computer control, making the operation of the instrument easy and convenient to use. The computer controls the instrument's drive system, the detector gain and checks to see that the sample load valve is in the right position. It is also programmed to assist in a simple calibration of the instrument. The instrument and software have been integrated into a system that is easy and convenient to use, leaving the operator free to concentrate on the data collection and analysis.
Data Collection and Analysis
KinTek Corporation is constantly upgrading the control computer and A/D converter to afford rapid data collection and analysis. Data fitting is accomplished by nonlinear regression. Data can be fit to one, two or three exponentials or to a burst equation.Data can also be fit by computer simulation to the reaction mechanism by global fitting methods using KinTek Explorer™.
Data can also be easily exported for import into the data fitting and graphics presentation program of your choice.
Computer Specifications: (as of 3/10/2009)
- MiniTower with Intel 3.16 GHz dual core processor
- 2 GHz RAM
- 19” widescreen LCD monitor
- Dual 250 GB SATA hard drives set up in RAID1 configuration
- Windows XP Professional OS
- 48×/32×/48× CD-RW/DVD Combo drive
Keep in mind that KinTek Corporation is constantly upgrading the computer that controls the stopped-flow instrument. The instrument is shipped with the latest model computer available.
Light Source for Optimal Signal
Optimal signal-to- noise is provided through several unique advances. A high intensity, stable arc lamp source illuminates the sample using a parabolic mirror and lens-free optics to provide optimal throughput to a monochromator. The optical path is free of lenses, which would limit light throughput. The electronic circuits have been designed to provide the most noise-free amplification possible.
Specifications
Wavelength range: 220 - 800 nm.Wavelength accuracy: 1 nm.
Stray light: less than 0.02%
Spectral bandwidth: Continuously variable slit width providing a bandwidth of 4 nm/mm of slit width.
Temperature range: 0 - 70° C range of temperature is maintained by circulating water bath (not provided).
Dead time: Less than 1 millisecond.
Syringe volume & range: 5 ml standard, 0.25, 0.5, 1.0, and 2.0 also available.
Computer operating system: Windows XP
A/D Converter: 416 kHz, 16-bit, 8 channels
Physical Dimensions
| Component | Width × Depth × Height | Weight |
|---|---|---|
| Stop-flow stand: | ||
| Motor controller | ||
| Light Source: | ||
| Lamp power supply: |