SF-E100 Stopped-Flow
Contents
Great Performance
The SF-E100 provides a research-grade stopped-flow instrument priced at the teaching level. It is a complete system including pneumatic drive, light source, PC and software for data collection and analysis.
Features
- Pneumatic Plus™ drive mechanism that combines the dual linear bearings with a pressure regulating solenoid to provide smooth and reliable continuous motion for reliable mixing and performance.
- Affordability without compromising performance.The SF-E100 affordable price allows for ownership of a research grade instrument at a fraction of the cost of comparable systems. Over time, the SF-E100 can be upgraded with a wide array of accessories.
- Sample Economy: As little as 30 µl per reactant is required and as little as 100µl of priming volume is needed.
- Ease of Use: Designing a system for teaching labs demands that ease of use considerations take a high priority. Students can generally start acquiring and analyzing data with as a little as 15 minutes of training.
- Small Footprint: The footprint of the SF-E100 has been designed to save laboratory bench space by combining the controller electronics with the syringe drive assembly.
- Choice: There are two SF-E100 models to choose from: For teaching labs the most affordable configuration is the SF-E100-A, (A-configuration), which has deuterium light source and a photodiode for absorbance experiments. For fluorescence applications, we recommend the SF-E100-F (F-configuration), which uses a super quiet Xenon light source and has both a photodiode and a photomultiplier tube. This configuration allows simultaneous fluorescence and absorbance detection.
Specifications
Syringe Drive Mechanism:
- No of Syringes: 2
- Minimum Sample Volume: 30 µl
- Priming Volume: 100µl per reactant
- Dead Time: 1.4 ms
- Syringe Mechanism: Computer-controlled Pneumatic Drive Plus™ technology provides precise and reproducible mixing of 2 solutions.
- Syringe Chamber & cell assembly: The syringe/cell assembly can be easily removed for maintenance or for changing cell types. The syringe chamber is transparent and can be thermostatted by a circulating water bath (provided separately).
- Syringe Volume and Range: 2.5 ml standard.
- Temperature Range: A range of 4°-70°C is maintained by a circulating water bath (provided separately).
- Observation Cells: We provide a choice of four observation cell path lengths: 2.0, 5.0, 10, and 25 mm. 5.0 mm is included with the instrument.
Light Source
- For absorbance work (A-Configuration): A 180-500nm high uniform deuterium source.
- For fluorescence work (F-configuration): A Super quiet 150W Xenon source for increased intensity and stability. The lamp is surrounded by a parabolic mirror to provide optimal collection of light intensity. The arc lamps must be cooled by a circulating water bath (provided separately). A highly regulated, current-controlled power supply and igniter provide stable light output (<0.1% ripple after warm-up).
Detectors
A UV-sensitive photomultiplier (optional) and a UV-enhanced photodiode (standard) are provided for simultaneous measurement of fluorescence and absorbance. A second (optional) photomultiplier can be used to provide an additional fluorescence channel for simultaneous measurements of two wavelengths or for real time polarization anisotropy measurements.
Data Collection and Analysis
413 kHz, 16-bit A/D converter provides fast and accurate data collection. Data can be fit to 1, 2 or 3 exponentials or 1, 2 exponentials plus a linear phase. Data are fit by nonlinear regression using the Marquat Method. Multiple traces can be averaged to improve signal-to-noise.
Computer
The following lists the minimum configuration of the computer provided with the instrument. KinTek Corporation is constantly updating the computer shipped with the SF-E100 stopped-flow instrument.
- Dell Precision T3500 MiniTower with Intel 2.53 GHz quad core processor
- 4 GHz RAM
- 1 GB ATI Fire Pro Graphics
- Dual 500 GB Hard Drives in a RAD1 configuration
- Windows 7 Professional OS
- 48×32×48× CD-RW/DVD Combo drive
- Dell Ultrasharp 20" widescreen LCD monitor
System Requirements
- Power Requirements: 110 volts, 15 amps.
- Compressed Air Requirement: ˜60 psi regulated source.
*Footprint |
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| Dimensions: | (Width × Depth × Height) | Weight: |
| Stopped-Flow Stand: | 36L × 21W × 41H cm | 72 kg |
*Deuterium Light source & Power Supply: |
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| Light Source: | 61L × 25W × 25H cm | 28 kg |
| Lamp Power Supply: | 30L × 20W × 20H cm | 18 kg |
*150W Xenon Light source & Power Supply: |
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| Light Source: | 24L × 23W × 20H cm | 46 kg |
| Lamp power supply: | 28L × 15W × 20H cm | 48 kg |